L4141

Sigma

 

Lectin from Anguilla anguilla (eel)

Protein ≥15 % by Lowry

MDL number:MFCD01191580

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Description

Analysis NoteAgglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg per mL sluiton. The activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol ActionsAnguilla anguilla agglutinin (AAA) has anti-H blood group specificity. The lectin has an affinity for α-L-fucosyl residues.
Preparation NotePurified by affinity chromatography
Physical formHighly purified, lyophilized powder containing a minimum 15% protein (Lowry); balance primarily NaCl.

Properties

formlyophilized powder
potency<20 μg per mL
compositionProtein, ≥15% Lowry
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

References

referenceHorejsi, V. and Kocourek, J. Biochim. Biophys. Acta 538, 299, (1978)
 Kelly, C. Biochem. J. 220, 221, (1984)